Prendre rendez-vous . They guarantee the specificity of the amplification reaction. These questions are being raised by many physicians around the world, not only in the United States but also in France, Belgium (Belgium Health Experts Demand Investigation Of WHO For Faking Coronavirus Pandemic), France, Germany, Italy, the United Kingdom, the United States and the United Kingdom. PARIS (Reuters) - Global airlines on Monday hit back at international bans on flights from Britain imposed over fears of a new COVID-19 strain, saying more testing could keep borders open. « Pour l’instant aucune mutation ou délétion n’a été associée à une perte de sévérité de la maladie sur une grande échelle géographique mais de nombreuses publications devraient bientôt préciser ces points. The main limitation (weakness) of the RT-PCR test, in the current pandemic situation, is its extreme sensitivity (false positive) if a suitable threshold of positivity (Ct) is not chosen. The Tests: The Achilles Heel of the COVID-19 House of Cards. : ... Courses. Corona, False Alarm? “The first test developed at La Charité in Berlin by Dr. Victor Corman and his associates in January 2020 allows to highlight the RNA sequences present in 3 genes of the virus called E, RdRp and N. To know if the sequences of these genes are present in the RNA samples collected, it is necessary to amplify the sequences of these 3 genes in order to obtain a signal sufficient for their detection and quantification. The lower the Ct used to detect the virus fragment, the higher the viral load is considered to be (high). This step precedes the amplification phase. Aged care facility information for COVID-19 testing. On the other hand, in certain clinical contexts or on the basis of specific evocative symptoms, there, yes, an electrocardiogram can be beneficial. In comparison, the human genome has more than 3 billion base pairs. Anyone Who Tells You V... Current advice from the Australian Commission on Safety and Quality in Health indicates patients presenting for elective surgery or investigations should be screened with a questionnaire prior to arranging the intervention, and then re-screening and checking body temperature on the day of the procedure. It makes it much less likely that they have COVID-19, but no test is perfect. Reactivation of COVID-19 is extremely unlikely. La saturation des laboratoires peut encore accroître ce risque et générer des faux positifs accidentels. For patients who are unable to receive request forms electronically: Perfluorinated chemical testing - PFAS - PFOS, Sullivan Nicolaides Pathology laboratories, Domestic travellers (including interstate). La personne est soit positive, soit négative. Des publications récentes soulignent que la dose détectable par PCR est inférieure à la dose infectieuse ou contagieuse : aucun virus infectieux n’a pu être retrouvé chez les patients asymptomatiques présentant des tests PCR positifs avec un Ct élevé. COVID-19 Press review. (7) qui ont décrit pour les génomes viraux disponibles jusqu’en mai 2020 un déplacement mutationnel sur la spike et le complexe de réplication vers des gènes codant pour d’autres protéines non structurelles qui interagissent avec les voies de défense de l’hôte, il semble que le taux de mutation du SARS-CoV-2 s’accélère depuis mai, impliquant principalement des mutations C vers U. L’augmentation du taux de mutation du SRAS-CoV-2 génère des génotypes viraux plus éloignés de la souche Wuhan initiale que ceux observés de mars à avril. [28] Le chômage de masse est désormais mondial. Tests de diagnostic ultra sensibles, les tests RT-PCR sortent positifs même pour des individus qui portent trop peu de virus pour être encore contagieux. L’échec de la stratégie actuelle est plutôt lié à sa conception naïve et inapplicable qu’aux mauvais comportements des citoyens. Here is an example of a laboratory result (approved by Sciensano, the Belgian national reference center) in an RT-PCR negative patient: In the NYT, experts compiled three datasets with officials from the states of Massachusetts, New York and Nevada that mention them. Is Covid-19 the Greatest Hoax in History? A serum sample in high-risk patients may also be collected for future antibody testing. And yet, all these people had to isolate themselves, with all the dramatic psychological and economic consequences, while they were not sick and probably not contagious at all. This is the starting postulate, the premise of all official propaganda, which justifies all restrictive government measures: isolation, confinement, quarantine, mandatory masks, color codes by country and travel bans, tracking, social distances in companies, stores and even, even more importantly, in schools [2]. He is a Research Associate of the  entre for Research on Globalization (CRG). Assessment of COVID-19 transmission in the Luxembourg population. Voir nos conditions générales d'utilisation. The RT-PCR test is a laboratory diagnostic technique that is not well suited to clinical medicine. Teams are continuously monitoring the evolution of the SARS-CoV-2 viral genome as it evolves [9-10-11], through the mutations it undergoes. Setting General paediatric department of a university hospital in Paris… Université de Paris enters the international university rankings . Some dare to find all this normal, or a lesser price to pay in the face of the pandemic of positive PCR tests. [24]. Si, comme on l’observe en ce moment, la diffusion virale reprend, faut-il dépister plus massivement ou revoir la stratégie de protection de la population ? It may also generate a 'false sense of security' in hospital staff assuming the patient cannot be infected with COVID-19. If there is a contraindication to the nasal approach, or preferably to the individual being tested, depending on the official organs, the oropharyngeal approach (through the mouth) is also acceptable. We do not guarantee that this is a comprehensive list, since the information below has been submitted voluntarily by test suppliers and is not independently verified. Ainsi, un petit Ct correspond à un grand nombre de copies, un grand Ct à un petit nombre de copies. In Massachusetts, between 85 and 90% of people who tested positive in July with a Ct of 40 would have been considered negative with a Ct of 30, adds the NYT. In the case of SARS-CoV-2, the element is a fragment of the viral genome, not the virus itself. When requesting pathology during a telehealth consultation, we suggest that you send the request form as an attachment by email to the patient who then prints the form prior to their arrival at a collection centre. With 1 copy/μl of a sample (Ct 35), without cough, without symptoms, one can understand why all these doctors and scientists say that a positive RT-PCR test means nothing, nothing at all in terms of medicine and clinic! We have not yet scheduled the vaccination of patients and for the time being are only proceeding with the vaccination of the health professionals concerned by Phase 1 of the national plan. Some set it at 37. My hope is that, finally, properly informed, more and more people will demand that this strategy be stopped, because it is all of us, enlightened, guided by real benevolence and common sense, who must decide our collective and individual destinies. This over-sensitivity of the RT-PCR test is deleterious and misleading! If you are concerned about a test result being a false-positive result, please contact our microbiologists (07) 3377 8534 to discuss the result. If a sputum sample is available, this should also be submitted. This Ct threshold must be informed with the positive RT-PCR result so that the physician knows how to interpret this positive result, especially in an asymptomatic person, in order to avoid unnecessary isolation, quarantine, psychological trauma. Buy New $14.00 Useful information. We are unable to perform any tests that are handwritten or entered as ‘free text’ on the request form. Air France has set up a partnership with the laboratories Synlab and Biogroup to assist customers who need to take the COVID-19 detection test requested by certain authorities before their departure. However, common sense gives obvious credence to the notion that the more virus a person has inside him or her, especially in the upper airways (oropharynx and nasopharynx), with symptoms such as coughing and sneezing, the higher the risk of contagiousness, proportional to the viral load and the importance of the person’s symptoms. False-negative tests are not common, but they have been reported. Place de l'Hôtel de Ville 75004 Paris. Even a symptomatic person should not automatically be tested, as long as they can place themselves in isolation for 7 days. Very few people, including doctors, understand how a PCR test works. Indeed, if we draw a straight line from the present events, if they are maintained, we could find ourselves once again confined with hundreds, thousands of human beings forced to remain inactive, which, for the professions of catering, entertainment, sales, fairgrounds, itinerants, canvassers, risks being catastrophic with bankruptcies, unemployment, depression, suicides by the hundreds of thousands. Today, our governments, supported by their scientific safety advice, are making us do the opposite and put the test first, followed by a clinical reflection necessarily influenced by this prior test, whose weaknesses we have just seen, particularly its hypersensitivity. in Germany, Spain…. An unusual stroll through the Père-Lachaise cemetery Le scientifique doit mesurer la grandeur des risques et ne pas se contenter d’affirmer qu’un événement adverse est « possible ». We can review the testing and repeat any testing as required. It is a binary, qualitative diagnostic technique that confirms (positive test) or not (negative test) the presence of an element in the medium being analyzed. Jean-Luc Gala (UCL) estime que les futures mesures de la Celeval, tel le lockdown, vont tuer l’économie, provoquer des suicides et déstabiliser l’État. While every care is taken with testing, false-positive results can rarely occur. At the beginning of the year, the SARS-CoV-2 genome was sequenced. Please note: State public health authorities require all requests for COVID-19 serological testing and all results to be notifiable. Mullis was referring to the AIDS virus (HIV retrovirus or HIV) [5], before the COVID-19 pandemic, but this opinion on the limitation of the technique in viral infections [6], by its creator, cannot be dismissed out of hand; it must be taken into account! » [14]. Discover the incomparable charm of the Montmartre district and its museums, vineyards,windmills, Sacré-Cœur…! According to them, the threshold (Ct) considered results in positive diagnoses in people who do not represent any risk of transmitting the virus! Translated from French by Global Research. ». Introduction: using a technique to lock down society All current propaganda on the COVID-19 pandemic is based on an assumption that is considered obvious, true and no longer questioned: Positive RT-PCR test means being sick with COVID. PhD, Pharmacien biologiste, ancien Chargé de Recherches INSERM, ancien Interne des Hôpitaux de Paris. Testing of asymptomatic, low-risk individuals prior to surgery is not recommended at a national level. Starting from a clinical situation with the presence or absence of particular symptoms in a patient, we consider different diagnoses based on tests. Vous pouvez joindre directement le laboratoire de votre choix sans avoir recours à notre service téléphonique, encart promotionnel indépendant. Is this a good idea? (as of 04:06 EDT - Details). Best Price: $36.69 Est-ce une bonne idée ? [12] https://nextstrain.org/, We are incorporating SARS-CoV-2 genomes as soon as they are shared and providing analyses and situation reports. [11] https://www.researchgate.net/profile/Helene_Banoun Evolution of SARS-CoV-2: Review of mutations, role of the host immune system, octobre 2020, mise à jour par Hélène Banoun. Test Coronavirus SARS-CoV-2 (COVID-19) CORONAVIRUS Gouvernement.lu. A virologist at the University of California admits that an RT-PCR test with a Ct greater than 35 is too sensitive. It is the opposite that guarantees quality medicine. Kary Mullis was awarded the Nobel Prize in Chemistry in 1993. The nucleic acid (DNA-RNA), the component of the genes, is a sequence of bases. Le dépistage de masse permet d'identifier les foyers d'infection (clusters) et de prendre les mesures sanitaires pour empêcher la propagation du virus. This is what underlies the sensitivity of the RT-PCR assay. 7 avril 2020, Laboratoire de biologie et pharmacologie appliquée (LBPA), Clémence Richetta, maître de conférences au département biologie de l’ENS Paris-Saclay et chercheuse en virologie au LBPA : https://www.youtube.com/watch?v=hNVDHCf8bGA, Former research fellow at INSERM (French Institute for Health and Medical Research), [16] Par Pierre Sonigo, virologiste (un des découvreurs du VIH), MD PhD, CSO at Sebia, clinical diagnostics, https://www.linkedin.com/pulse/diagnostic-du-covid19-comprendre-les-tests-pcr-leur-et-pierre-sonigo/?trackingId=pTYxDkpvRzKHWZwCzxSIag%3D%3D, Diagnostic du COVID19 : comprendre les tests PCR, leur interprétation et leurs limites, publié le 16 septembre 2020. Décryptage. As I said at the beginning of the article, in medicine we always start from the person: we examine him/her, we collect his/her symptoms (complaints-anamnesis) and objective clinical signs (examination) and on the basis of a clinical reflection in which scientific knowledge and experience intervene, we make diagnostic hypotheses.